Review



ca17 2a12 fitc bio rad rat anti dog cd5  (Bio-Rad)


Bioz Verified Symbol Bio-Rad is a verified supplier
Bioz Manufacturer Symbol Bio-Rad manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Bio-Rad ca17 2a12 fitc bio rad rat anti dog cd5
    Figure 3 | Phenotypic changes of purified CD3+CD5dimCD21− cells during culture for 21 days. (A) CD3+CD5dimCD21− cells were purified from freshly isolated PBMCs using a cell sorter. The purity of CD3+CD5dimCD21− cells after sorting was more than 98% (n = 5). (B) Phenotypic analysis of the purified CD3+CD5dimCD21− cells during culture for 21 days. <t>CD3−CD5−CD21−</t> cells were proliferated from purified CD3+CD5dimCD21− cells, and the majority of expanded cells in culture were CD3−CD5−CD21− after 21 days. The phenotype of most of these expanded CD3−CD5−CD21− cells was CD4− CD8+/− after 21 days of stimulation. The results shown are representative results from one of five different donors. (C) The rate of proliferation was calculated by counting the total number of viable cells in culture. Values represent the mean ± SD (n = 5).
    Ca17 2a12 Fitc Bio Rad Rat Anti Dog Cd5, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ca17+2a12+fitc+bio+rad+rat+anti+dog+cd5/Rat+anti+Dog+CD5/pm29755462-49-66-68
    Average 93 stars, based on 23 article reviews
    ca17 2a12 fitc bio rad rat anti dog cd5 - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Comparison of Phenotypic and Functional Characteristics Between Canine Non-B, Non-T Natural Killer Lymphocytes and CD3 + CD5 dim CD21 - Cytotoxic Large Granular Lymphocytes."

    Article Title: Comparison of Phenotypic and Functional Characteristics Between Canine Non-B, Non-T Natural Killer Lymphocytes and CD3 + CD5 dim CD21 - Cytotoxic Large Granular Lymphocytes.

    Journal: Frontiers in immunology

    doi: 10.3389/fimmu.2018.00841

    Figure 3 | Phenotypic changes of purified CD3+CD5dimCD21− cells during culture for 21 days. (A) CD3+CD5dimCD21− cells were purified from freshly isolated PBMCs using a cell sorter. The purity of CD3+CD5dimCD21− cells after sorting was more than 98% (n = 5). (B) Phenotypic analysis of the purified CD3+CD5dimCD21− cells during culture for 21 days. CD3−CD5−CD21− cells were proliferated from purified CD3+CD5dimCD21− cells, and the majority of expanded cells in culture were CD3−CD5−CD21− after 21 days. The phenotype of most of these expanded CD3−CD5−CD21− cells was CD4− CD8+/− after 21 days of stimulation. The results shown are representative results from one of five different donors. (C) The rate of proliferation was calculated by counting the total number of viable cells in culture. Values represent the mean ± SD (n = 5).
    Figure Legend Snippet: Figure 3 | Phenotypic changes of purified CD3+CD5dimCD21− cells during culture for 21 days. (A) CD3+CD5dimCD21− cells were purified from freshly isolated PBMCs using a cell sorter. The purity of CD3+CD5dimCD21− cells after sorting was more than 98% (n = 5). (B) Phenotypic analysis of the purified CD3+CD5dimCD21− cells during culture for 21 days. CD3−CD5−CD21− cells were proliferated from purified CD3+CD5dimCD21− cells, and the majority of expanded cells in culture were CD3−CD5−CD21− after 21 days. The phenotype of most of these expanded CD3−CD5−CD21− cells was CD4− CD8+/− after 21 days of stimulation. The results shown are representative results from one of five different donors. (C) The rate of proliferation was calculated by counting the total number of viable cells in culture. Values represent the mean ± SD (n = 5).

    Techniques Used: Purification, Isolation

    Figure 6 | Comparison of the proliferative capacity in CD3−CD5−CD21− non-B, non-T (CD5−), CD3+CD5dimCD21− (CD5dim), and CD3+CD5brightCD21− (CD5bright) lymphocytes. PBMCs were stained with Violet Cell Trace Dye before culture. To evaluate the proliferation of the different lymphocyte subsets, cells were stimulated with IL-15; IL-2; and IL-15; canine NK cell-sensitive canine thyroid adenocarcinoma (CTAC) cells; CTAC cells and IL-15; CTAC cells, IL-2, and IL-15; or concanavalin A for 7 days. Cells cultured in medium alone served as a negative control. Results are representative of data from five different donors. The percentage of proliferating cells within the respective subsets is indicated.
    Figure Legend Snippet: Figure 6 | Comparison of the proliferative capacity in CD3−CD5−CD21− non-B, non-T (CD5−), CD3+CD5dimCD21− (CD5dim), and CD3+CD5brightCD21− (CD5bright) lymphocytes. PBMCs were stained with Violet Cell Trace Dye before culture. To evaluate the proliferation of the different lymphocyte subsets, cells were stimulated with IL-15; IL-2; and IL-15; canine NK cell-sensitive canine thyroid adenocarcinoma (CTAC) cells; CTAC cells and IL-15; CTAC cells, IL-2, and IL-15; or concanavalin A for 7 days. Cells cultured in medium alone served as a negative control. Results are representative of data from five different donors. The percentage of proliferating cells within the respective subsets is indicated.

    Techniques Used: Comparison, Staining, Cell Culture, Negative Control

    Related Articles

    Flow Cytometry:

    Article Title: Comparison of Phenotypic and Functional Characteristics Between Canine Non-B, Non-T Natural Killer Lymphocytes and CD3 + CD5 dim CD21 - Cytotoxic Large Granular Lymphocytes.
    Article Snippet: .. Based on these results, the interrelationship between these two cell populations as putative canine NK cells was assumed, and we hypothesized that phenotypic modulation might occur between TaBle 1 | Antibodies used for flow cytometry in this study. antibody clone conjugates supplier Primary antibody (isotype) Mouse anti Human CD14 (IgG2a, κ)* M5E2 PE-Cy7 BD Pharmingen Mouse anti-Canine CD21 (IgG1) CA2.1D6 RPE Bio-Rad Mouse anti-Dog CD3 (IgG1) CA17.2A12 FITC Bio-Rad Rat anti-Dog CD5 (IgG2a) YKIX322.3 APC Bio-Rad Rat anti-Dog CD4 (IgG2a) YKIX302.9 RPE Bio-Rad Rat anti-Dog CD8 (IgG1) YCATE55.9 RPE Bio-Rad Mouse anti-Canine CD21 (IgG1) CA2.1D6 – Bio-Rad Mouse anti-Dog CD3 (IgG1) CA17.2A12 – Bio-Rad Mouse anti-Dog CD11c (IgG1) CA11.6A1 – Bio-Rad Mouse anti-Dog CD11d (IgG1) CA11.8H2 – Bio-Rad Canine TCRαβ (IgG1) CA15.8G7 – Perter Moorea Canine TCRγδ (IgG2a) CA20.8H1 – Perter Moorea intracellular staining Mouse anti Human Ki-67 (IgG1, kappa)* 20Raj1 PE-Cyanine7 Invitrogen Mouse anti Human Granzyme B (IgG1, κ)** GB11 PE BD Pharmingen Mouse anti Human EOMES (IgG1, kappa)b WD1928 PE-Cyanine7 Invitrogen Mouse anti Human/Mouse T-bet (IgG1, kappa)*** eBio4B10 (4B10) PE Invitrogen isotype control Mouse IgG1 Negative Control – FITC Bio-Rad Mouse IgG1 kappa Isotype Control P3.6.2.8.1 PE-Cyanine7 Invitrogen Mouse IgG1 kappa Isotype Control P3.6.2.8.1 PE Invitrogen Mouse IgG1, κ Isotype Control MOPC-21 PE BD Pharmingen secondary antibody Goat anti-Mouse IgG (H + L) Secondary Antibody – Pacific Blue Invitrogen Cross reactivity to the canine equivalent reported* by the supplier. ..

    Staining:

    Article Title: Comparison of Phenotypic and Functional Characteristics Between Canine Non-B, Non-T Natural Killer Lymphocytes and CD3 + CD5 dim CD21 - Cytotoxic Large Granular Lymphocytes.
    Article Snippet: .. Based on these results, the interrelationship between these two cell populations as putative canine NK cells was assumed, and we hypothesized that phenotypic modulation might occur between TaBle 1 | Antibodies used for flow cytometry in this study. antibody clone conjugates supplier Primary antibody (isotype) Mouse anti Human CD14 (IgG2a, κ)* M5E2 PE-Cy7 BD Pharmingen Mouse anti-Canine CD21 (IgG1) CA2.1D6 RPE Bio-Rad Mouse anti-Dog CD3 (IgG1) CA17.2A12 FITC Bio-Rad Rat anti-Dog CD5 (IgG2a) YKIX322.3 APC Bio-Rad Rat anti-Dog CD4 (IgG2a) YKIX302.9 RPE Bio-Rad Rat anti-Dog CD8 (IgG1) YCATE55.9 RPE Bio-Rad Mouse anti-Canine CD21 (IgG1) CA2.1D6 – Bio-Rad Mouse anti-Dog CD3 (IgG1) CA17.2A12 – Bio-Rad Mouse anti-Dog CD11c (IgG1) CA11.6A1 – Bio-Rad Mouse anti-Dog CD11d (IgG1) CA11.8H2 – Bio-Rad Canine TCRαβ (IgG1) CA15.8G7 – Perter Moorea Canine TCRγδ (IgG2a) CA20.8H1 – Perter Moorea intracellular staining Mouse anti Human Ki-67 (IgG1, kappa)* 20Raj1 PE-Cyanine7 Invitrogen Mouse anti Human Granzyme B (IgG1, κ)** GB11 PE BD Pharmingen Mouse anti Human EOMES (IgG1, kappa)b WD1928 PE-Cyanine7 Invitrogen Mouse anti Human/Mouse T-bet (IgG1, kappa)*** eBio4B10 (4B10) PE Invitrogen isotype control Mouse IgG1 Negative Control – FITC Bio-Rad Mouse IgG1 kappa Isotype Control P3.6.2.8.1 PE-Cyanine7 Invitrogen Mouse IgG1 kappa Isotype Control P3.6.2.8.1 PE Invitrogen Mouse IgG1, κ Isotype Control MOPC-21 PE BD Pharmingen secondary antibody Goat anti-Mouse IgG (H + L) Secondary Antibody – Pacific Blue Invitrogen Cross reactivity to the canine equivalent reported* by the supplier. ..

    Control:

    Article Title: Comparison of Phenotypic and Functional Characteristics Between Canine Non-B, Non-T Natural Killer Lymphocytes and CD3 + CD5 dim CD21 - Cytotoxic Large Granular Lymphocytes.
    Article Snippet: .. Based on these results, the interrelationship between these two cell populations as putative canine NK cells was assumed, and we hypothesized that phenotypic modulation might occur between TaBle 1 | Antibodies used for flow cytometry in this study. antibody clone conjugates supplier Primary antibody (isotype) Mouse anti Human CD14 (IgG2a, κ)* M5E2 PE-Cy7 BD Pharmingen Mouse anti-Canine CD21 (IgG1) CA2.1D6 RPE Bio-Rad Mouse anti-Dog CD3 (IgG1) CA17.2A12 FITC Bio-Rad Rat anti-Dog CD5 (IgG2a) YKIX322.3 APC Bio-Rad Rat anti-Dog CD4 (IgG2a) YKIX302.9 RPE Bio-Rad Rat anti-Dog CD8 (IgG1) YCATE55.9 RPE Bio-Rad Mouse anti-Canine CD21 (IgG1) CA2.1D6 – Bio-Rad Mouse anti-Dog CD3 (IgG1) CA17.2A12 – Bio-Rad Mouse anti-Dog CD11c (IgG1) CA11.6A1 – Bio-Rad Mouse anti-Dog CD11d (IgG1) CA11.8H2 – Bio-Rad Canine TCRαβ (IgG1) CA15.8G7 – Perter Moorea Canine TCRγδ (IgG2a) CA20.8H1 – Perter Moorea intracellular staining Mouse anti Human Ki-67 (IgG1, kappa)* 20Raj1 PE-Cyanine7 Invitrogen Mouse anti Human Granzyme B (IgG1, κ)** GB11 PE BD Pharmingen Mouse anti Human EOMES (IgG1, kappa)b WD1928 PE-Cyanine7 Invitrogen Mouse anti Human/Mouse T-bet (IgG1, kappa)*** eBio4B10 (4B10) PE Invitrogen isotype control Mouse IgG1 Negative Control – FITC Bio-Rad Mouse IgG1 kappa Isotype Control P3.6.2.8.1 PE-Cyanine7 Invitrogen Mouse IgG1 kappa Isotype Control P3.6.2.8.1 PE Invitrogen Mouse IgG1, κ Isotype Control MOPC-21 PE BD Pharmingen secondary antibody Goat anti-Mouse IgG (H + L) Secondary Antibody – Pacific Blue Invitrogen Cross reactivity to the canine equivalent reported* by the supplier. ..

    Negative Control:

    Article Title: Comparison of Phenotypic and Functional Characteristics Between Canine Non-B, Non-T Natural Killer Lymphocytes and CD3 + CD5 dim CD21 - Cytotoxic Large Granular Lymphocytes.
    Article Snippet: .. Based on these results, the interrelationship between these two cell populations as putative canine NK cells was assumed, and we hypothesized that phenotypic modulation might occur between TaBle 1 | Antibodies used for flow cytometry in this study. antibody clone conjugates supplier Primary antibody (isotype) Mouse anti Human CD14 (IgG2a, κ)* M5E2 PE-Cy7 BD Pharmingen Mouse anti-Canine CD21 (IgG1) CA2.1D6 RPE Bio-Rad Mouse anti-Dog CD3 (IgG1) CA17.2A12 FITC Bio-Rad Rat anti-Dog CD5 (IgG2a) YKIX322.3 APC Bio-Rad Rat anti-Dog CD4 (IgG2a) YKIX302.9 RPE Bio-Rad Rat anti-Dog CD8 (IgG1) YCATE55.9 RPE Bio-Rad Mouse anti-Canine CD21 (IgG1) CA2.1D6 – Bio-Rad Mouse anti-Dog CD3 (IgG1) CA17.2A12 – Bio-Rad Mouse anti-Dog CD11c (IgG1) CA11.6A1 – Bio-Rad Mouse anti-Dog CD11d (IgG1) CA11.8H2 – Bio-Rad Canine TCRαβ (IgG1) CA15.8G7 – Perter Moorea Canine TCRγδ (IgG2a) CA20.8H1 – Perter Moorea intracellular staining Mouse anti Human Ki-67 (IgG1, kappa)* 20Raj1 PE-Cyanine7 Invitrogen Mouse anti Human Granzyme B (IgG1, κ)** GB11 PE BD Pharmingen Mouse anti Human EOMES (IgG1, kappa)b WD1928 PE-Cyanine7 Invitrogen Mouse anti Human/Mouse T-bet (IgG1, kappa)*** eBio4B10 (4B10) PE Invitrogen isotype control Mouse IgG1 Negative Control – FITC Bio-Rad Mouse IgG1 kappa Isotype Control P3.6.2.8.1 PE-Cyanine7 Invitrogen Mouse IgG1 kappa Isotype Control P3.6.2.8.1 PE Invitrogen Mouse IgG1, κ Isotype Control MOPC-21 PE BD Pharmingen secondary antibody Goat anti-Mouse IgG (H + L) Secondary Antibody – Pacific Blue Invitrogen Cross reactivity to the canine equivalent reported* by the supplier. ..



    Similar Products

    ca17 2a12 fitc bio rad rat anti dog cd5  (Bio-Rad)
    93
    Bio-Rad ca17 2a12 fitc bio rad rat anti dog cd5
    Figure 3 | Phenotypic changes of purified CD3+CD5dimCD21− cells during culture for 21 days. (A) CD3+CD5dimCD21− cells were purified from freshly isolated PBMCs using a cell sorter. The purity of CD3+CD5dimCD21− cells after sorting was more than 98% (n = 5). (B) Phenotypic analysis of the purified CD3+CD5dimCD21− cells during culture for 21 days. <t>CD3−CD5−CD21−</t> cells were proliferated from purified CD3+CD5dimCD21− cells, and the majority of expanded cells in culture were CD3−CD5−CD21− after 21 days. The phenotype of most of these expanded CD3−CD5−CD21− cells was CD4− CD8+/− after 21 days of stimulation. The results shown are representative results from one of five different donors. (C) The rate of proliferation was calculated by counting the total number of viable cells in culture. Values represent the mean ± SD (n = 5).
    Ca17 2a12 Fitc Bio Rad Rat Anti Dog Cd5, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ca17+2a12+fitc+bio+rad+rat+anti+dog+cd5/Rat+anti+Dog+CD5/pm29755462-49-66-68
    Average 93 stars, based on 1 article reviews
    ca17 2a12 fitc bio rad rat anti dog cd5 - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 3 | Phenotypic changes of purified CD3+CD5dimCD21− cells during culture for 21 days. (A) CD3+CD5dimCD21− cells were purified from freshly isolated PBMCs using a cell sorter. The purity of CD3+CD5dimCD21− cells after sorting was more than 98% (n = 5). (B) Phenotypic analysis of the purified CD3+CD5dimCD21− cells during culture for 21 days. CD3−CD5−CD21− cells were proliferated from purified CD3+CD5dimCD21− cells, and the majority of expanded cells in culture were CD3−CD5−CD21− after 21 days. The phenotype of most of these expanded CD3−CD5−CD21− cells was CD4− CD8+/− after 21 days of stimulation. The results shown are representative results from one of five different donors. (C) The rate of proliferation was calculated by counting the total number of viable cells in culture. Values represent the mean ± SD (n = 5).

    Journal: Frontiers in immunology

    Article Title: Comparison of Phenotypic and Functional Characteristics Between Canine Non-B, Non-T Natural Killer Lymphocytes and CD3 + CD5 dim CD21 - Cytotoxic Large Granular Lymphocytes.

    doi: 10.3389/fimmu.2018.00841

    Figure Lengend Snippet: Figure 3 | Phenotypic changes of purified CD3+CD5dimCD21− cells during culture for 21 days. (A) CD3+CD5dimCD21− cells were purified from freshly isolated PBMCs using a cell sorter. The purity of CD3+CD5dimCD21− cells after sorting was more than 98% (n = 5). (B) Phenotypic analysis of the purified CD3+CD5dimCD21− cells during culture for 21 days. CD3−CD5−CD21− cells were proliferated from purified CD3+CD5dimCD21− cells, and the majority of expanded cells in culture were CD3−CD5−CD21− after 21 days. The phenotype of most of these expanded CD3−CD5−CD21− cells was CD4− CD8+/− after 21 days of stimulation. The results shown are representative results from one of five different donors. (C) The rate of proliferation was calculated by counting the total number of viable cells in culture. Values represent the mean ± SD (n = 5).

    Article Snippet: Based on these results, the interrelationship between these two cell populations as putative canine NK cells was assumed, and we hypothesized that phenotypic modulation might occur between TaBle 1 | Antibodies used for flow cytometry in this study. antibody clone conjugates supplier Primary antibody (isotype) Mouse anti Human CD14 (IgG2a, κ)* M5E2 PE-Cy7 BD Pharmingen Mouse anti-Canine CD21 (IgG1) CA2.1D6 RPE Bio-Rad Mouse anti-Dog CD3 (IgG1) CA17.2A12 FITC Bio-Rad Rat anti-Dog CD5 (IgG2a) YKIX322.3 APC Bio-Rad Rat anti-Dog CD4 (IgG2a) YKIX302.9 RPE Bio-Rad Rat anti-Dog CD8 (IgG1) YCATE55.9 RPE Bio-Rad Mouse anti-Canine CD21 (IgG1) CA2.1D6 – Bio-Rad Mouse anti-Dog CD3 (IgG1) CA17.2A12 – Bio-Rad Mouse anti-Dog CD11c (IgG1) CA11.6A1 – Bio-Rad Mouse anti-Dog CD11d (IgG1) CA11.8H2 – Bio-Rad Canine TCRαβ (IgG1) CA15.8G7 – Perter Moorea Canine TCRγδ (IgG2a) CA20.8H1 – Perter Moorea intracellular staining Mouse anti Human Ki-67 (IgG1, kappa)* 20Raj1 PE-Cyanine7 Invitrogen Mouse anti Human Granzyme B (IgG1, κ)** GB11 PE BD Pharmingen Mouse anti Human EOMES (IgG1, kappa)b WD1928 PE-Cyanine7 Invitrogen Mouse anti Human/Mouse T-bet (IgG1, kappa)*** eBio4B10 (4B10) PE Invitrogen isotype control Mouse IgG1 Negative Control – FITC Bio-Rad Mouse IgG1 kappa Isotype Control P3.6.2.8.1 PE-Cyanine7 Invitrogen Mouse IgG1 kappa Isotype Control P3.6.2.8.1 PE Invitrogen Mouse IgG1, κ Isotype Control MOPC-21 PE BD Pharmingen secondary antibody Goat anti-Mouse IgG (H + L) Secondary Antibody – Pacific Blue Invitrogen Cross reactivity to the canine equivalent reported* by the supplier.

    Techniques: Purification, Isolation

    Figure 6 | Comparison of the proliferative capacity in CD3−CD5−CD21− non-B, non-T (CD5−), CD3+CD5dimCD21− (CD5dim), and CD3+CD5brightCD21− (CD5bright) lymphocytes. PBMCs were stained with Violet Cell Trace Dye before culture. To evaluate the proliferation of the different lymphocyte subsets, cells were stimulated with IL-15; IL-2; and IL-15; canine NK cell-sensitive canine thyroid adenocarcinoma (CTAC) cells; CTAC cells and IL-15; CTAC cells, IL-2, and IL-15; or concanavalin A for 7 days. Cells cultured in medium alone served as a negative control. Results are representative of data from five different donors. The percentage of proliferating cells within the respective subsets is indicated.

    Journal: Frontiers in immunology

    Article Title: Comparison of Phenotypic and Functional Characteristics Between Canine Non-B, Non-T Natural Killer Lymphocytes and CD3 + CD5 dim CD21 - Cytotoxic Large Granular Lymphocytes.

    doi: 10.3389/fimmu.2018.00841

    Figure Lengend Snippet: Figure 6 | Comparison of the proliferative capacity in CD3−CD5−CD21− non-B, non-T (CD5−), CD3+CD5dimCD21− (CD5dim), and CD3+CD5brightCD21− (CD5bright) lymphocytes. PBMCs were stained with Violet Cell Trace Dye before culture. To evaluate the proliferation of the different lymphocyte subsets, cells were stimulated with IL-15; IL-2; and IL-15; canine NK cell-sensitive canine thyroid adenocarcinoma (CTAC) cells; CTAC cells and IL-15; CTAC cells, IL-2, and IL-15; or concanavalin A for 7 days. Cells cultured in medium alone served as a negative control. Results are representative of data from five different donors. The percentage of proliferating cells within the respective subsets is indicated.

    Article Snippet: Based on these results, the interrelationship between these two cell populations as putative canine NK cells was assumed, and we hypothesized that phenotypic modulation might occur between TaBle 1 | Antibodies used for flow cytometry in this study. antibody clone conjugates supplier Primary antibody (isotype) Mouse anti Human CD14 (IgG2a, κ)* M5E2 PE-Cy7 BD Pharmingen Mouse anti-Canine CD21 (IgG1) CA2.1D6 RPE Bio-Rad Mouse anti-Dog CD3 (IgG1) CA17.2A12 FITC Bio-Rad Rat anti-Dog CD5 (IgG2a) YKIX322.3 APC Bio-Rad Rat anti-Dog CD4 (IgG2a) YKIX302.9 RPE Bio-Rad Rat anti-Dog CD8 (IgG1) YCATE55.9 RPE Bio-Rad Mouse anti-Canine CD21 (IgG1) CA2.1D6 – Bio-Rad Mouse anti-Dog CD3 (IgG1) CA17.2A12 – Bio-Rad Mouse anti-Dog CD11c (IgG1) CA11.6A1 – Bio-Rad Mouse anti-Dog CD11d (IgG1) CA11.8H2 – Bio-Rad Canine TCRαβ (IgG1) CA15.8G7 – Perter Moorea Canine TCRγδ (IgG2a) CA20.8H1 – Perter Moorea intracellular staining Mouse anti Human Ki-67 (IgG1, kappa)* 20Raj1 PE-Cyanine7 Invitrogen Mouse anti Human Granzyme B (IgG1, κ)** GB11 PE BD Pharmingen Mouse anti Human EOMES (IgG1, kappa)b WD1928 PE-Cyanine7 Invitrogen Mouse anti Human/Mouse T-bet (IgG1, kappa)*** eBio4B10 (4B10) PE Invitrogen isotype control Mouse IgG1 Negative Control – FITC Bio-Rad Mouse IgG1 kappa Isotype Control P3.6.2.8.1 PE-Cyanine7 Invitrogen Mouse IgG1 kappa Isotype Control P3.6.2.8.1 PE Invitrogen Mouse IgG1, κ Isotype Control MOPC-21 PE BD Pharmingen secondary antibody Goat anti-Mouse IgG (H + L) Secondary Antibody – Pacific Blue Invitrogen Cross reactivity to the canine equivalent reported* by the supplier.

    Techniques: Comparison, Staining, Cell Culture, Negative Control